Analyse des mecanismes de reparation des cassures simple-brin de l'ADN // Analysis of single-strand DNA break repair
ABG-125495
ADUM-58316 |
Thesis topic | |
2024-08-23 |
Ecole normale supérieure
Paris - France
Analyse des mecanismes de reparation des cassures simple-brin de l'ADN // Analysis of single-strand DNA break repair
Reparation de l'ADN, PARylation, piege magnetique
DNA repair, PARylation, magnetic trapping
DNA repair, PARylation, magnetic trapping
Topic description
Le projet consiste a decortiquer la voie de reparation des cassures simple-brin de l'ADN telle que mise en place chez les humains. Il consistera en l'analyze par différentes modalités molécule-unique les interactions entre les protéines eukaryotes PARP1, XRCC1 et Ligase 3alpha au cours de la localization de la cassure d'ADN et sa réparation, ainsi qu'au cours du désassemblage final du complexe de réparation. Les modalités employées inclueront des expériences de spectroscopie de force par piège magnétique, et des expériences de fluorescence molécule-unique. Ainsi une vue intégrée des activités précatalytiques, catalytiques, et post-catalytiques de cette voie de réparation essentielle sera développée.
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The project consists of dissecting the repair pathway for DNA single-strand breaks as established in humans. It will consist in the analysis by different single-molecule modalities of the interactions between the eukaryotic proteins PARP1, XRCC1 and Ligase 3alpha during the localization of the DNA break and its repair, as well as during the final disassembly of the DNA complex. repair. The modalities employed will include magnetic trap force spectroscopy experiments, and single-molecule fluorescence experiments. Thus an integrated view of the precatalytic, catalytic, and post-catalytic activities of this essential repair pathway will be developed.
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Début de la thèse : 01/10/2024
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The project consists of dissecting the repair pathway for DNA single-strand breaks as established in humans. It will consist in the analysis by different single-molecule modalities of the interactions between the eukaryotic proteins PARP1, XRCC1 and Ligase 3alpha during the localization of the DNA break and its repair, as well as during the final disassembly of the DNA complex. repair. The modalities employed will include magnetic trap force spectroscopy experiments, and single-molecule fluorescence experiments. Thus an integrated view of the precatalytic, catalytic, and post-catalytic activities of this essential repair pathway will be developed.
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Début de la thèse : 01/10/2024
Funding category
Funding further details
Programmes gouvernementaux hors France et Union Européenne
Presentation of host institution and host laboratory
Ecole normale supérieure
Institution awarding doctoral degree
Ecole normale supérieure
Graduate school
657 Sciences du Vivant
Candidate's profile
Master's en biologie ou equivalent.
Master's in Biology or equivalent.
Master's in Biology or equivalent.
2024-08-31
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