Use of liposomes to optimize the cryopreservation of ram semen
ABG-125388 | Sujet de Thèse | |
02/08/2024 | Financement public/privé |
- Biologie
- Biochimie
- Biotechnologie
Description du sujet
The success of cryopreservation is based on maintaining the functional integrity of cells, despite the stresses induced by the freezing/thawing processes. However, currently, some cells of interest are still poorly resistant to these stresses. In rams, for example, after freezing and insemination, spermatozoa are no longer able to travel up the female genital tract to fertilize the oocyte. One explanation could be linked to the lipid composition of sperm membranes, which makes them particularly sensitive to freezing. Our hypothesis is that the use of liposomes, innovative lipid nanovectors, acting at the level of the plasma membrane of cells, could promote their resistance to freezing. The objectives of the thesis are: 1) to acquire elements of understanding on the "lipid weaknesses" of ram spermatozoa and on its interactions with cryoprotectants (fluorescent labeling techniques in confocal microscopy); 2) to synthesize liposomes of optimized composition, specifically adapted to ram spermatozoa (modulation of the composition of the lipid bilayer of liposomes and encapsulation of antioxidant molecules); 3) to evaluate the cryoprotective effects of these liposomes in vitro (flow cytometry, heterologous In Vitro Fertilization) then in vivo, on different sheep breeds.
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Présentation établissement et labo d'accueil
UPSP 2021.A104 - ICE (Cell-Environment Interactions) VetAgro Sup Veterinary Campus of Lyon.
Occasional travel for field experiments.
Profil du candidat
Master Degree in Biotechnologies or Biosciences.
Dedicated interest in Cell biology and strong scientific curiosity.
High motivation, reliability and initiative.
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CASDEN
Aérocentre, Pôle d'excellence régional
Nokia Bell Labs France
Groupe AFNOR - Association française de normalisation
ONERA - The French Aerospace Lab
MabDesign
CESI
TotalEnergies
ADEME
Tecknowmetrix
Généthon
SUEZ
Institut Sup'biotech de Paris
Institut de Radioprotection et de Sureté Nucléaire - IRSN - Siège
Ifremer
MabDesign
PhDOOC
Laboratoire National de Métrologie et d'Essais - LNE
ANRT
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Sujet de ThèseRef. ABG125548Centre de Recherche en CardioVasculaire et Nutrition (C2VN) - INSERM 1263 - INRAE 1260 - Aix-Marseille universitéMarseille - Provence-Alpes-Côte d'Azur - France
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Biologie -
EmploiCDIRef. ABG125568Cours GalienStrasbourg - Grand Est - France
Résponsable pédagogique études médicales
Biologie - Santé, médecine humaine, vétérinaireNiveau d'expérience indifférent -
EmploiCDIRef. ABG123642Laboratoire des Courses Hippiques (GIE LCH)Verrières-le-Buisson - Ile-de-France - France
Chargé(e) de Recherche et Innovation (H/F) / Senior Scientist Research & Innovation (M/F)
Chimie - BiochimieConfirmé